Jul 14 – 19, 2024
Georgia State University College of Law
America/New_York timezone
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Phylogeographic patterns of Escherichia coli isolated from white-tailed deer, Odocoileus virginianus, during necropsy in farmed deer surveillance program

Jul 15, 2024, 4:20 PM
20m
Knowles Conference Center/Second Level-242 - Room 242 (Georgia State University College of Law)

Knowles Conference Center/Second Level-242 - Room 242

Georgia State University College of Law

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Paper Infectious Diseases Paper Presentations

Speaker

Ms Morgan Metrailer (Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida)

Description

White-tailed deer (Odocoileus virginianus) (WTD) farms are distributed throughout Florida and number nearly 400. The Cervidae Health Research Initiative (CHeRI) is an initiative that investigates disease and health of farmed cervids statewide. Within the Florida industry, Epizootic Hemorrhagic disease virus (EHDV) and Bluetongue virus (BTV) are major causes of disease and associated economic losses. Often, deer survive EHDV or BTV infections but succumb to secondary bacterial infections, including Escherichia coli. E. coli can be opportunistic with severe illness or death occurring in hosts weakened from viral infection. We investigated presumptive E. coli strains isolated from WTD during necropsy investigations. Genomic DNA was extracted and whole genome sequenced from 61 suspect E. coli isolates. Exploratory spatial data analysis (ESDA) of the distribution of E. coli phylotypes, distribution of E. coli, and deer between ranches was performed. We examined phylogenetic relationships between isolates, categorized by ranch and year and compared to all reported E. coli isolates in Florida from EnteroBase. One strain was Enterobacter hormaechei and the other 60 were E. coli. Two strains were toxigenic. Deer isolates represented 7 phylogroups with B1 being the most prevalent (45/60) and geographically widespread (14/16 counties reporting E. coli). Phylogroup A was the second most prevalent phylogroup. In at least two instances two phylogroups were present within a single animal. We found that deer isolates spanned many of known phylotypes in Florida from animals and humans. Diversity patterns suggest animals are infected locally with most animals on ranches having high genomic similarity within phylogroups.

Primary authors

Ms An Chi Cheng (Department of Large Animal Clinical Sciences, University of Florida) Mr Andrew Bluhm (Emerging Pathogens Institute, University of Florida) Mr Austin Surphlis (Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida) Dr Jason Blackburn (Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida) Dr Juan Campos Krauer (College of Veterinary Medicine & Department of Large Animal Clinical Sciences, University of Florida) Dr Kuttichantran Subramaniam (Department of Infectious Diseases and Immunology, College of Veterinary Medicine, University of Florida) Dr Kwangcheol Casey Jeong (Emerging Pathogens Institute & Department of Animal Sciences, University of Florida) Dr Michael Norris (School of Life Sciences, University of Hawaii at Manoa, Honolulu) Ms Morgan Metrailer (Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida) Dr Samantha Wisely (Department of Wildlife Ecology and Conservation, University of Florida) Dr Treenate Jiranantasak (Emerging Pathogens Institute, University of Florida)

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